Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA
نویسندگان
چکیده
منابع مشابه
Coamplification of HIV-1 proviral DNA and viral RNA in assays used for quantification of HIV-1 RNA.
Elevated HIV-1 viral load (VL) observed in specimens frozen in situ in plasma preparation tubes (PPTs) compared to EDTA plasma specimens may affect therapeutic monitoring of HIV-infected patients. The increase in viral load is cell associated and minimized when plasma from the PPT is aspirated or recentrifuged prior to freezing. This study investigates the contribution of integrated HIV-1 provi...
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Abstract Background and Objectives In Iran, Khorasan province is an endemic area for HTLV-1 virus. Considering the inability of serological tests to determine HTLV-1 in window period, their failure to confirm the indetermination results of western blot, and given the probability for HTLV-1 transfusion transmission, a SYBR green-based Real Time PCR was set to measure the HTLV-1 proviral load. ...
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ژورنال
عنوان ژورنال: Methods
سال: 2021
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2021.05.006